. Block diagram of an HPLC–MS. A three ingredient combination enters the HPLC. When component A elutes with the column, it enters the MS ion source and ionizes to variety the parent ion and a number of other fragment ions.. Solvent triangle for optimizing a reversed-period HPLC separation. The 3 blue circles demonstrate cellular phases consisting
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The key parts of a HPLC are shown in Figure (PageIndex 3 ). The role of a pump should be to power a liquid (cell phase) by means of at a selected stream fee (milliliters per moment). The injector serves to introduce the liquid sample to the flow stream on the mobile period. Column is easily the most central and significant element of HPLC, as well
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